cDNA cloning and phylogenetic analysis of growth hormone genes from the Mullet Mugil platanus (Mugilomorpha, Mugilidae) and the halfbeak Hemiramphus brasiliensis (Atherinomorpha, Hemiramphidae)
DOI:
https://doi.org/10.5088/atlântica.v28i2.796Keywords:
Atherinomorpha, Hormônio do crescimento, Filogenia, Growth hormone, Mugilomorpha, PhylogenyAbstract
Atherinid and mugilid fishes are currently considered sister groups. However, there are many controversies about this hypothesis. In order to evaluate the phylogenetic relationship between aterinids, mugilids and percomorphs, GH (growth hormone) cDNA sequences of the mullet Mugil platanus (Mugilomorpha) and the halfbeak Hemiramphus brasiliensis (Atherinomorpha) were obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The H. brasiliensis GH cDNA contains an open reading frame of 615 nucleotides encoding a preprotein of 204 amino acid residues, while the partial M. platanus GH cDNA contains an open reading frame of 597 nucleotides encoding a preprotein of 199 amino acid residues. Both hormones exhibit typical GH features when compared to those of mature GHs from other Acanthopterygian fishes. The deduced GH amino acid sequences were used to infer a phylogenetic tree using the maximum parsimony method. The topology found has placed Atherinomorpha and Mugilomorpha within Percomorpha, indicating that mugilids are probably more related to perciforms than atherinids.Downloads
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Published
2011-03-26
How to Cite
Meier, K. M., Castaño, C., Laurino, J. P., Sabaj, J. A. L., & Marins, L. F. F. (2011). cDNA cloning and phylogenetic analysis of growth hormone genes from the Mullet Mugil platanus (Mugilomorpha, Mugilidae) and the halfbeak Hemiramphus brasiliensis (Atherinomorpha, Hemiramphidae). Atlântica (Rio Grande), 28(2), 97–102. https://doi.org/10.5088/atlântica.v28i2.796
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