Porcentages phagocytosis’ evaluation uva irradiation effects of haemocytes and glucose’s concentration haemolymph in pink-shrimp Farfantepenaeus paulensis

Abstract

The crustacean are naturally exposed to ultraviolet radiation, it can produce biochemical and in immune function changes and predispose to diseases manifestation. Objective: To quantify the haemolymph glucose concentration and evaluate the phagocitary index of haemocytes in shrimp Farfantepenaeus paulensis exposed to UVA irradiation. Methodology: 21 shrimps were divided in one control group and 3 treatment groups exposed to UVA radiation for 1, 5 and 10 days. Phagocytosis was carried out in slide glass, the haemocytes capacity to engulf heat-killed yeast (Saccharomyces cerevisae) were analyzed. Glucose was determined by enzymatic method (oxidase glucose). The results were expressed by mean ± standard deviations and analyzed by ANOVA follow Tuckey test. Results: Phagocytosis (%) and the glucose concentration (mmol/l) of the animals exposed for 1, 5 and 10 days and control group were respectively, 71.70±2.03 and 0.36±0.19; 70.0±4.73 and 0.88±0.47; 63.80±3.67 and 0.18±0.03 and 85.65±2.81 and 2.41±0.74. Control group showed higher phagocytosis index (P>0.05) and the exposition during 10 days impaired the index (P>0.05). It was observed lower concentration of glucose in groups exposed than to control group (P>0.05). Conclusion: The results reached with the hypothesis of immunosuppression by the exposition to UVA.